Study on the Surface Interactions of Co(II) with Phospholipids from the Marine Environment

Natural phospholipid (PL)n extract from cultured green algae Dunaliela tertiolecta was used as the main component of cell membranes for studies on interaction with trace metal Co(II). The phospholipids were extracted other biological components using TiO2-µSPE cartridges selective phosphate group according to a completely new protocol. Co(II) natural and standard phospholipids, phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylglycerol (PG) are registered only in presence additional chelating ligand 1,10-Phenathroline (Phen). 1,10-Phenathroline, model humic substances marine environment, formed neutral complex by substitution water molecules central ions. hydrophobic mixed-ligand complexes enabled remaining coordination shell Co(II), which voltammetric measurements. Co(II)-Phen-PL is reduced adsorbed state at −1.65 V transfer two electrons, followed its irreversible dissociation desorption, indicating an EC mechanism. between Co(II)-Phen-lipids confirmed atomic force microscopy (AFM). AFM images PL, PL Phen mixture showed different 3D structures mica surface, changes caused cobalt(II), 1,10-Phenanthroline phospholipids.